RNA

Part:BBa_K2715042:Design

Designed by: Daniel Partridge   Group: iGEM18_Nottingham   (2018-10-12)


Synthetic guide sequence for S. pyrogenes cas9 - targeting sequence


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This is a synthetic guide RNA fragment minus the specific 20bp targeting region, designed to work with the Cas9 protein from S. pyrogenes. This part was natively Biobrick compatible and so did not require modification.

Source

The synthetic guide RNA was sourced from vectors described in the following publication (see references below)

References

Huang, H., Chai, C., Li, N., Rowe, P., Minton, N.P., Yang, S., Jiang, W. and Gu, Y., 2016. CRISPR/Cas9-based efficient genome editing in Clostridium ljungdahlii, an autotrophic gas-fermenting bacterium. ACS synthetic biology, 5(12), pp.1355-1361.